anti il 2rβ  (Bio X Cell)

Journal: Cell Reports Medicine

Article Title: Targeting severe acidity for tumor-activatable Interleukin-2 therapy

doi: 10.1016/j.xcrm.2025.102572

Figure Lengend Snippet: pH-responsive blocking and restoration of IL-2 receptor binding by UPS 5.3 (A) Schematic of the experimental design to assess the intrinsic interaction between UPS 5.3 micelles and proteins. (B) FPLC chromatograms showing individual proteins or UPS 5.3 /protein mixtures. (C) Molecular docking model of the DBA dimer (blue) with IL-2, highlighting predicted interaction sites. (D–F) Biolayer interferometry analysis of IL-2-Fc binding to IL-2Rα, IL-2Rβ, and IL-2Rγ, with and without UPS 5.3 NP encapsulation. (G) Schematic illustrating ex vivo assessment of IL-2-Fc binding to immune cells from various organs. (H) Quantification of IL-2-Fc binding to lymphocytes isolated from liver, lung, spleen, and tumor ( n = 5). Data are presented as mean ± SEM. See also and .

Article Snippet: InVivoMAb anti-mouse CD122 (IL-2Rβ) , BioXcell , Cat# BE0298 RRID: AB_2687820.

Techniques: Blocking Assay, Binding Assay, Encapsulation, Ex Vivo, Isolation

Journal: Current Issues in Molecular Biology

Article Title: IL-2 Receptor Expression in Renal Cell Carcinoma Cells: IL-2 Influences Cell Survival and Induces Cell Death

doi: 10.3390/cimb47100830

Figure Lengend Snippet: Expression of IL-2Rα, IL-2Rβ, and IL-2Rγ subunits in renal cell carcinoma cells, demonstrated by PCR products on an agarose gel. Representative blot with relative quantification shows IL-2Rα, IL-2Rβ, and IL-2Rγ protein detection in A-498, ACHN, Caki-1, and Caki-2 cancer cell lines . PBMCs: Peripheral Blood Mononuclear Cells, which are the control cells.

Article Snippet: IL-2Rβ (C-10): sc-393093 , 1:200 , Santa Cruz Biotechnology, Heidelberg, Germany.

Techniques: Expressing, Agarose Gel Electrophoresis, Quantitative Proteomics, Control

Journal: Current Issues in Molecular Biology

Article Title: IL-2 Receptor Expression in Renal Cell Carcinoma Cells: IL-2 Influences Cell Survival and Induces Cell Death

doi: 10.3390/cimb47100830

Figure Lengend Snippet: Protein expression of IL-2Rα, IL-2Rβ, and IL-2Rγ subunits in renal cell carcinoma cells, visualized by Western blot analysis. Representative blot shows IL-2Rα , IL-2Rβ , and IL-2Rγ protein detection in A-498, ACHN, Caki-1, and Caki-2 cancer cell lines. Jurkat cells are the control cells.

Article Snippet: IL-2Rβ (C-10): sc-393093 , 1:200 , Santa Cruz Biotechnology, Heidelberg, Germany.

Techniques: Expressing, Western Blot, Control

Journal: Current Issues in Molecular Biology

Article Title: IL-2 Receptor Expression in Renal Cell Carcinoma Cells: IL-2 Influences Cell Survival and Induces Cell Death

doi: 10.3390/cimb47100830

Figure Lengend Snippet: Representative flow cytometry histograms of the IL-2Rγ and IL-2Rβ surface expression for the four RCC cell lines tested. Graphs ( A – D ) show IL-2Rγ expression, and graphs ( E – H ) show IL-2Rβ expression (dark blue curves) with isotype controls (red curves) and unstaining controls (gray curves) in A-498, ACHN, Caki-1, and Caki-2 cell lines.

Article Snippet: IL-2Rβ (C-10): sc-393093 , 1:200 , Santa Cruz Biotechnology, Heidelberg, Germany.

Techniques: Flow Cytometry, Expressing